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In specific embodiments, the invention may be embodied in whole or in part as software recorded on this fixed media. The DNA strands in the buffer were first annealed by raising the temperature to degrees C. Labels can be attached to the oligonucleotides or PNAs either during automated synthesis or by post-synthetic reactions which have been described before see, e. MegAlign typically does not provide a summary of the percent identity between two sequences. Other reactive fluorophore derivatives and their respective sites of attachment include the succinimidyl ester of 5-carboxyrhodamine-6G RHD coupled to an amino group; an iodoacetamide of tetramethylrhodamine coupled to a sulphydryl group; an isothiocyanate of tetramethylrhodamine coupled to an amino group; or a sulfonylchloride of Texas red coupled to a sulphydryl group.
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